Cloning and functional characterization of Arabidopsis thaliana d-amino acid aminotransferase - d-aspartate behavior during germination
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چکیده
منابع مشابه
Biological role of D-amino acid oxidase and D-aspartate oxidase. Effects of D-amino acids.
D-Amino acids administered to animals are absorbed by the intestine and transported through the blood-stream to solid tissues where they are oxidized in vivo by D-amino acid oxidase and D-aspartate oxidase to produce the same compounds they do in vitro; i.e. NH3, H2O2, and the keto acid corresponding to the amino acid ingested. In the liver and kidneys of the animals, an inverse relationship ex...
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In this study, gene encoding Aspartate aminotransferase was cloned from Arabidopsis thaliana by RT-PCR, named F23N and its bioinformatics analysis was performed. It was found that there is one pyridoxal-phosphate attachment site at 250-270 (SYAKnmGLyGERIG) and other domains, furthermore, the transmembrane helices was not found in F23N, suggesting that F23N encoding product might not belong to m...
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Aspartate: 2-oxoglutarate aminotransferase from the anaerobic protozoon Trichomonas vaginalis was purified to homogeneity and characterized. It is a dimeric protein of overall Mr approx. 100000. Only a single isoenzyme was found in T. vaginalis. The overall molecular and catalytic properties have features in common with both the vertebrate cytoplasmic and mitochondrial isoenzymes. The purified ...
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cDNA and genomic clones encoding DNA topoisomerase I were isolated from Arabidopsis thaliana Xgtl1 and XFix libraries by low stringency hybridization with a Saccharomyces cerevisiae TOPi probe. The cDNA clones include a 2748-base pair open reading frame predicting an amino acid sequence that is highly homologous to sequences encoded by TOP1 from yeast and human sources. The sequence of the upst...
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The activity of aspartate aminotransferase a vitamin B6 containing enzyme was measured in the laysed synaptosomes prepared from rat brain, during development. Administration of vitamin B6 increased the enzyme activity throughout development. The stimulating effect of this vitamin was prevented when the animals were previously treated with D-penicillamine. However, penicillamine alone had n...
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ژورنال
عنوان ژورنال: FEBS Journal
سال: 2008
ISSN: 1742-464X
DOI: 10.1111/j.1742-4658.2008.06279.x